In Vitro Regeneration of Banana Cv. Nanjanagud Rasabale (AAB) by Shoot Tip Culture

The cultivar Nanjanagud Rasabale (Musa spp. AAB, Silk subgroup) geographical indication tagged and leading cultivar of Mysore district. It is known for unique taste, has a huge demand across the country. In vitro regeneration in banana is preferred for faster multiplication rate compared to sucker propagation. The present study was carried out with the objective to investigate the effect of different antioxidants, BAP and auxins on in vitro regeneration of banana cv. Nanjanagud Rasabale. Shoot tip explants cultured on MS liquid medium supplemented with ascorbic acid 50 mg/l was found effective in controlling browning. Enhanced shoot proliferation and shoot growth were observed with MS medium containing BAP 4.0 mg/l + NAA0.50 mg/l. In vitro rooting of microshoots was effective with half strength MS medium supplied with NAA 0.5 mg/l.