Effectual Gold Nanoprobe Sensor for Screening Cow Milk Adulteration in Goat Milk—Comparison With Conventional PCR

Ortiz, Jose Manuel Llopis and Tsouknidas, Christos and Pytel, Sophie and Papatheodorou, Spiridon and Vougiouklaki, Despina and Tsakni, Aliki and Antonopoulos, Dionysiοs and Tsakali, Efstathia and Impe, Jan Van and Houhoula, Dimitra (2021) Effectual Gold Nanoprobe Sensor for Screening Cow Milk Adulteration in Goat Milk—Comparison With Conventional PCR. Journal of Agricultural Science, 13 (5). p. 41. ISSN 1916-9752

[thumbnail of 6073ab3eeeeca.pdf] Text
6073ab3eeeeca.pdf - Published Version

Download (847kB)

Abstract

Different methods have been used to detect milk adulteration, but in recent years the use of nanomaterials has been introduced as an interesting tool, due to their optical properties. A gold nanoparticle (AuNP) probe application was developed in order to evaluate milk adulteration. The methodology relies on the colorimetric differentiation and the participation profiles of the hybridization DNA sequence with the AuNPs. Various concentrations, from 0.01 (traces) to 50%, of cows’ milk in goats’ milk samples were prepared for DNA extraction, further identification with the AuNPs and comparison with a conventional PCR. Also, a total of 40 dairy products from goat milk, commonly consumed in Greece, were tested. Negative and goat reaction mixtures showed a purplish coloured solution with a peak at > 570 nm, while samples containing bovine DNA had an absorbance closer to the characteristic peak of the AuNPs at 520-525 nm. Presence of bovine milk was detected even at traces level, achieving a detection level comparable to those achieved by conventional PCR. The use of AuNPs in milk products, provides a low-cost and easy-to-perform method and offers the possibility to detect fraudulent practices in various food matrices.

Item Type: Article
Subjects: Archive Paper Guardians > Agricultural and Food Science
Depositing User: Unnamed user with email support@archive.paperguardians.com
Date Deposited: 09 May 2023 10:00
Last Modified: 18 Mar 2024 03:53
URI: http://archives.articleproms.com/id/eprint/887

Actions (login required)

View Item
View Item