Antibacterial and Ftir Spectral Analysis of Methanolic Extract of Gliricidia sepium Leaves

Aim: The present study was carried out with the objective to analyse the methanol extracts of Gliricidia sepium leaves using Fourier transform infrared (FTIR) spectroscopic method.

Place and Period of Study: The study was carried out at the Department of Microbiology Federal University of Technology Akure between January 2013 and July 2014.

Methodology: The in vitro killing rate effect (antibacterial activities) of the extract- bioactive components was examined to see if they have inhibitory effect on the selected test organisms using pour plating method. The FTIR method was performed on a spectrophotometer system which detects different characteristic peak values associated with various functional groups present the extracts.

Results: All the isolates were susceptible to the extract with the number of the test organisms drastically reduced with time. The FTIR analysis of methanol extracts of G. sepium confirmed the presence of alcohols, aldehydes, alkanes, amide, alkenes, aromatics, carboxylic acids, ketones, esters, ethers, phenols, primary amines, Aliphatic bromo compound, Aryl disulphide and aliphatic amines compounds, which showed at different peaks. The FTIR method was performed on a spectrophotometer system, which was used to determine the characteristic peak values and their functional groups.

Conclusion: The results of the present study generated the FTIR spectrum profile for the medicinally important plants showed that the leaf extract of G. sepium can be used for pharmacological purpose.