Comparative Antimicrobiotic Efficacy Test of Triple Antibiotic Paste, Double Antibiotic Paste with Fungicide and Calcium Hydroxide with Chitosan as Vehicle against Enterococcus faecalis: An In vitro Study

Introduction: This present research aimed to assess the in vitro antimicrobial efficacy of triple antibiotic paste and calcium hydroxide with two different vehicles against Enterococcus faecalis (E. faecalis).

Materials and Methodology: An agar well diffusion assay was used to determine the experimental medicaments' efficacy against E. faecalis. Medicaments were divided into six groups, which includes Triple antibiotic powder (TAP) with saline or chitosan, Double antibiotic powder with fungicide (DAP 1) with saline or Chitosan, and calcium hydroxide with saline or Chitosan. These medicines were tested in an agar well diffusion test for three days, i.e., 1,4,7 days. The diameters of growth inhibition zones were recorded and compared for each group were tested in an agar well diffusion test for three days, i.e., 1,4,7 days. The diameters of growth inhibition zones were recorded and compared for each group. These medicaments were evaluated for three days an agar well diffusion test, i.e., on 1,4,7 days. The inhibition zones diameters for each group were recorded and compared — the differences between groups analyzed by Kruskal-Wallis and Mann-Whitney U tests.

Results: The largest inhibition zones were observed for the triple antibiotic powder with chitosan and the smallest for Ca(OH)2 with saline. As days progress, they produced lesser antibacterial effects in all groups.

Conclusion: Triple antibiotic paste and Chitosan were more effective in eliminating microorganisms than calcium hydroxide and DAP 1.