In-vitro Antibacterial Activity of Hydroethanolic Leaf, Stem, Root Extract of Acalypha indica - A Comparative Study

Background and Aim: Acalypha indica is a weedy, short lived and monoecious plant. It has an antioxidant effect and it must be implemented to control the disease where free radicals are involved. Acalypha plant is useful for the bronchitis, pneumonia, pulmonary tuberculosis patients. A. indica plant is used in the rejuvenation in the worst conditions which may undergo infections in the microorganisms and it is the chemotherapeutic agent and distributed in the large contributions in human health and well being. The main aim of this study is to assess which part of A. indica has an antibacterial activity.

Materials and Methods: Agar well diffusion method was used for assessing the antimicrobial activity of the plant extract. The nutrient broth is inoculated with bacterial strains E. faecalis. The broth was then incubated at 37°C overnight. Antibacterial activity was determined by measurement of the diameter of zones of inhibition (mm).

Results: When compared to root, stem and leaves of hydroethanolic extract of A. indica, leaf maximum inhibition against E. faecalis. As the concentration of extract increases from 25 µg/ml to 100 µg/ml, the zone of inhibition also increases. This shows the antimicrobial activity is better at higher concentration.

Conclusion: A. indica has effective antimicrobial activity. It reduces the growth of various human pathogenic bacteria. The root, stem and leaf of A. indica possess Antibacterial activity against E. faecalis. leaf extract has significant antibacterial activity.