Cordyceps sinensis Acts As an Adenosine A3 Receptor Agonist on Mouse Melanoma and Lung Carcinoma Cells, and Human Fibrosarcoma and Colon Carcinoma Cells

Yoshikawa, Noriko and Nishiuchi, Arisa and Kubo, Erika and Yamaguchi, Yu and Kunitomo, Masaru and Kagota, Satomi and Shinozuka, Kazumasa and Nakamura, Kazuki (2011) Cordyceps sinensis Acts As an Adenosine A3 Receptor Agonist on Mouse Melanoma and Lung Carcinoma Cells, and Human Fibrosarcoma and Colon Carcinoma Cells. Pharmacology & Pharmacy, 02 (04). pp. 266-270. ISSN 2157-9423

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Abstract

Cordyceps sinensis, a parasitic fungus on the larva of Lapidoptera, has been used as a traditional Chinese medicine. We previously reported that the growth of B16-BL6 mouse melanoma (B16-BL6) cells and mouse Lewis lung carcinoma (LLC) cells was inhibited by cordycepin (3’-deoxyadenosine), an ingredient of Cordyceps sinensis, and its effect was antagonized by MRS1191, a selective adenosine A3 receptor (A3-R) antagonist although adenosine (up to 100 μM) had no effect on the growth of B16-BL6 and LLC cells. In this study, we investigated whether water extracts of Cordyceps sinensis (WECS) inhibit the growth of B16-BL6 cells, LLC cells, HT1080 human fibrosarcoma (HT1080) cells and CW-2 human colon carcinoma (CW-2) cells via their A3-R. As a result, the growth of all cell lines were potently inhibited by WECS (10 μg/mL) and the inhibitory effect of WECS was significantly antagonized by MRS1191 (1 μM). Furthermore, WECS included 2.34% w/w cordycepin and 0.12% w/w adenosine as components according to the HPLC- ECD system. In conclusion, WECS inhibited the proliferation of four cancer cell lines by stimulation of A3-R and the main component in WECS with anticancer action might be cordycepin instead of adenosine.

Item Type: Article
Subjects: Archive Paper Guardians > Chemical Science
Depositing User: Unnamed user with email support@archive.paperguardians.com
Date Deposited: 04 Mar 2023 12:36
Last Modified: 16 Feb 2024 04:17
URI: http://archives.articleproms.com/id/eprint/270

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