EFFECT OF HUMAN BLOOD SERUM ON SEALING ABILITY OF WHITE AND GRAY MTA, AN Ex vivo STUDY

Mineral trioxide aggregate (MTA) is an endodontic cement composed of tricalcium silicate, dicalcium silicate, tricalcium aluminate and bismuth oxide. The aim of the present study was to evaluate the effect of two different storage media on sealing ability of white and gray mineral trioxide aggregate (WMTA, GMTA). Forty single rooted extracted human teeth were selected. After canal preparation, root resection and root-end preparation, the teeth were randomly divided into two experimental groups, and root-end cavities were filled with WMTA and GMTA, respectively. Samples in each group were randomly subdivided into 10 teeth stored in either deionized water or human blood serum. Microleakage was measured using a fluid transport device. Using repeated measures test, microleakage was demonstrated to be significantly lower in human blood serum environment (P<0.001), although there was no significant difference between the two retrograde materials. It can be concluded that the type of storage medium could affect the Ex vivo sealing ability of MTA. In order to obtain closer results to clinical conditions, it is suggested to store MTA filled teeth in a medium similar to blood serum solution in Ex vivo studies.