Effects of Metosartan on Testis Tissue Chromatin and Enzyme Kinetics of Testicular RNaseA

Metosartan is the drug normally used as antihypertensive agent that act on heart and reduces
hypertension in humans. It’s components telmisartan is a angiotensin type I receptor blocker known to
induce apoptosis in urinary bladder and metoprolyl is the beta receptor blocker. RNase A is the most
experimental protein in the 20th century. Disulphide bonds are necessary for enzymatic action of
many proteins and it is also required for this protein. RNaseA kinetic studies is performed with the
drug metosartan using RNA as the substrate Metosartan is an combination of metoprolol and
telmisartan and found to contain inhibitory property on RNaseA. Protein degradation and thiol titration
assay has found to be that the drug has reducing property on RNaseA. The study concludes that
metosartin inhibits the activity of RNAse A allosterically by reducing the disulphide bonds present on
it. The reduction of disulphides may dissociate the enzyme to its monomer which is not highly active
hence reducing the catalytic activity of RNase A. HPLC studies confirm the RNaseA presence in
testes and drug also induce toroid packing of DNA in immature sperms.